Serodiagnosis of tuberculosis by enzyme immunoassay using A60 antigen
نویسندگان
چکیده
منابع مشابه
Enzyme-linked immunosorbent assay using Mycobacterium tuberculosis antigen 5 and PPD for the serodiagnosis of tuberculosis.
Enzyme-linked immunosorbent assays (ELISA) of immunoglobulin G antibody to Mycobacterium tuberculosis antigen 5 and tuberculin purified protein derivative (PPD) were assessed for the serodiagnosis of tuberculosis in 41 patients with active tuberculosis, 19 patients with inactive tuberculosis, and 59 healthy control subjects. Patients with active tuberculosis were studied serially at monthly int...
متن کاملDetection of poliovirus antigen by enzyme immunoassay.
A solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Rabbit and guinea pig antisera for the assay were raised against purified poliovirus type 3/Fin (strain 3/Fin/K) isolated from a fecal specimen from a meningitis patient during an outbreak of poliomyelitis in Finland in 1984. The EIA was highly specific for poliovirus type 3, and it was about 30 times ...
متن کاملEnzyme immunoassay for carcinoembryonic antigen.
In this rapid, simple enzyme immunoassay for carcinoembryonic antigen (CEA) in serum the "sandwich" system is used. The CEA is first extracted from the serum into acetate buffer by heating at 70 degrees C, then incubated in a polyvinyl microtitration plate previously coated with anti-CEA. The bound CEA is then reacted with anti-CEA conjugated to horseradish peroxidase and the activity of the pe...
متن کاملValue of ELISA IgG and IgM Antibodies using A60 Antigen in the Diagnosis of Tuberculosis
Objectives: To evaluate a new Modified Anda-TB IgG ELISA test for the serodiagnosis of Mycobacterium tuberculosis in patients and controls. Settings and duration: The study was carried out between January to December 2010 at the Microbiology Department, Umm Al-Qura University, Makkah. Patients and Methods: The Anda IgG and IgM ELISA tests were run on 674 patient’s sera which included 378 active...
متن کاملEvaluation of serological tests using A60 antigen for diagnosis of tuberculosis.
Identification of acid-fast bacilli (AFB) in sputum or tissue samples is among definite diagnostic methods of tuberculosis. However, this method of diagnosis is restricted by certain limitations. Serologic diagnosis of tuberculosis (TB) has been used for a long time. The aim of this study was to determine the sensitivity and, specificity of Antigen 60 (A60) IgG, IgA, IgM test results in TB diag...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2001
ISSN: 1198-743X
DOI: 10.1046/j.1198-743x.2001.00263.x